Translational_Unit

Part:BBa_K3525003:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_high-school   (2020-10-18)


T7 pro-His-ER sensor L-His-T7 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 923
    Illegal BglII site found at 1420
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1845
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add T7 promoter, His tag and T7 terminator to express the sensor protein in the E.coli.


Source

Rubing Liang, Jing Zhou. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Journal of Biotechnology. Vol.136/S(2008)S1-S772 Jing Zhou, Rubing Liang, Wenhua Wang. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Environmental Science and Technology 4th International Symposium on Novel Carbon Resource Sciences. 2009.

References